Short Communication (Published online: 08-12-2022)
TROUBLE SHOOTING THE EXTRACTION OF DNA FROM AVIAN BLOOD SAMPLES WITH A SOLID PHASE EXTRACTION KIT
Stella Cyriac, Junaid N, Vineetha P.G, Nimmy P and Pramod S
jivaonline, 135-139
Stella Cyriac, Junaid N, Vineetha P.G, Nimmy P and Pramod S: College of Avian Sciences & Management, Thiruvazhamkunnu, Palakkad, Kerala
Abstract
Solid phase DNA isolation kit was used to isolate genomic DNA (gDNA) from duck blood samples. The manufacturer’s protocol was modified by reducing the quantity of blood from 200 µL to 10 µL. Normal saline was used to extend the volume to 200 µL. DNA was isolated with the modified protocol and was used for amplifying a segment of exon 2 of duck growth hormone gene.
Keywords : Trouble shooting, DNA isolation, Silica membrane-based kit
Share this article on [Facebook] [LinkedIn]
Article history: Received: 18-05-2022, Accepted : 10-10-2022, Published online: 08-12-2022
Corresponding author: Pramod S
