Research Articles (Published online: 30-12-2023)
RECOVERY OF CARBAPENEM RESISTANT ENDOMETRIAL BACTERIAL ISOLATES FROM POSTPARTUM ENDOMETRITIS IN DAIRY CATTLE
Samiksha Agrawal, Ajay Pratap Singh, Rashmi Singh, Shyama N Prabhu, Jitendra Agrawal
jivaonline, 20-33
Samiksha Agrawal: College of Biotechnology, U.P. Pandit Deen Dayal Upadhyayay PashuChikitsa Vigyan Vishwavidhyalaya Ewam GoAnusandhan Sansthan Mathura, 281001 U.P.
Ajay Pratap Singh: Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry
U.P. Pandit Deen Dayal Upadhyayay PashuChikitsa Vigyan Vishwavidhyalaya Ewam GoAnusandhan Sansthan Mathura, 281001 U.P
Rashmi Singh: Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry
U.P. Pandit Deen Dayal Upadhyayay PashuChikitsa Vigyan Vishwavidhyalaya Ewam GoAnusandhan Sansthan Mathura, 281001 U.P
Shyama N Prabhu: 4Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry
U.P. Pandit Deen Dayal Upadhyayay PashuChikitsa Vigyan Vishwavidhyalaya Ewam GoAnusandhan Sansthan Mathura, 281001 U.P
Jitendra Agrawal: Department of Animal Reproduction and Gynecology, College of Veterinary Science and Animal Husbandry
U.P. Pandit Deen Dayal Upadhyayay PashuChikitsa Vigyan Vishwavidhyalaya Ewam GoAnusandhan Sansthan Mathura, 281001 U.P.
In this report, we describe recovery of multiple drug resistance organism resistant to most of the antibiotics with coexistence of ESBL, Carbapenam and AmpC ß-lactamase genes isolated from the bovine clinical endometritisin the Mathura region of India.Sampling involvesuterine discharge collected from 70 postpartum cattle diagnosed with metritis. Out of 62 bacterial isolates recovered, seven were showing carbapenamase resistant phenotype baseddisc diffusion test.MIC testing and phenotypic detection methodconfirmed resistance to imepenam for isolates of Pseudomonas aeruginosa (n=1),E. coli (n=2) and Enterobacter agglomerance (n=1). Multiplex PCR based genotyping confirmed presence Ambler class B Carbapenemase (VIM and IMP), along with TEM and CTX-M type ESBL respectively. All four isolates were phenotypically confirmed as AmpC β-lactamase producers.
Keywords : 5Department of Animal Reproduction and Gynecology
