Article Details Date: 30-12-2023 12:00:00

Research Articles (Published online: 30-12-2023)
RAW MILK AS A RESERVOIR OF ANTIBIOTIC RESISTANT ENTEROPATHOGENIC ESCHERICHIA COLI
Devika D. Sunil, Binsy Mathew, Latha. C, Deepa Jolly, Radha K.
jivaonline, 51-60
Devika D. Sunil: MVSc Scholar, Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651
Binsy Mathew: Assistant Professor, Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651
Latha. C: 3Director of Academics and Research, Kerala Veterinary and Animal Sciences University, Kerala, India
Deepa Jolly: Assistant Professor, Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651
Radha K.: Associate Professor, Department of Dairy Sciences and Technology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651

Abstract

Escherichia coli is a commensal in the intestine of humans and warm-blooded animals. Usually, they are harmless except certain strains like enteropathogenic, enteroinvasive, enteroaggregative, enterotoxigenic, enterohaemorrhagic and diffusely adherent E. coli. The present study was conducted to study the prevalence antibiotic resistant enteropathogenic Escherichia coli (EPEC) from raw cow milk in Ernakulam district of Kerala in India. A total of 100 raw cow milk samples were collected from various milk societies of the district. Escherichia coli was isolated by conventional culture technique on MacConkey agar. The culture and biochemical positive isolates were further confirmed by molecular technique by targeting genus specific 16S rRNA gene. The isolates obtained were further subjected to molecular confirmation by targeting eaeA and bfpA genes specific for enteropathogenic E. coli. The antibiotic sensitivity pattern of the confirmed isolates was tested against commonly used antibiotics by disc diffusion assay. Further molecular characterisation of the isolates was carried out by targeting dfrA1, dfrA10 for trimethoprim resistance, sul1 and sul2 for sulphamethoxazole resistance, and aadA1 and aadA2 for streptomycin resistance by Polymerase chain reaction (PCR).

Keywords : E. coli, raw cow milk, AMR

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Article history: Received: 03-12-2023, Accepted : 26-12-2023, Published online: 30-12-2023

Corresponding author: Binsy Mathew