Abstract

Extensive and indiscriminate use of antibiotics in treatment and control of mastitis may lead to emergence of antibiotic resistant bacteria and transfer of resistant genes. Among various pathogens, E. coli and Staphylococcus contributes to major occurrence of mastitis in dairy animals and also show antimicrobial resistance. Combination of phytochemicals with antimicrobial agents is shown to be effective in overcoming antimicrobial resistance. Methanolic extracts of Tectona grandis and Biophytum sensitivum are phytochemicals used in this study in combination with tetracycline in Escherichia coli, Klebsiella sp. and Staphylococcus aureus isolates from mastitis. In the present study disc diffusion antimicrobial assay by Kirby Bauer assay was conducted in E. coli, Klebsiella sp and S. aureus isolates from mastitis with tetracycline and methanolic extracts of Tectona grandis and Biophytum sensitivum combination at different doses and compared their efficacies.

Keywords : Antimicrobial activity, Biophytum sensitivum, Tectona grandis, Phytochemical screening