Abstract

The gastrointestinal tract of poultry is densely populated with microorganisms, which are presumed to interact with the host and ingested feed. CARI Nirbheek is a native chicken developed at the Desi unit of the institute, CARI, Izatnagar.  The birds are developed for backyard rearing system and are characterized by higher genetic resistance against diseases. In this experiment, these birds are reared under intensive system, which is entirely different from the backyard system of rearing. Metagenomic analysis of the gut microbiome of CARI Nirbheek revealed that Firmicute was the dominant phylum followed by Proteobacteria. Bacilli was the dominant class followed by Clostridia. Lactobacillus, Faecalibacterium and Enterococcus were the dominant genera. Modern techniques like NGS analysis of intestinal micro biome using primers targeting V 3 , V 4 and V 4 -V 6 region of 16S rRNA helped in revealing the microbial diversity of native chicken, CARI Nirbheek . The beneficial bacterial strains can be isolated and used as a potential probiotic for broiler.

Keywords : Arun. T. S*. Saxena V.K and Vineetha P.G