Abstract

Immunogenicity prediction of decellularised pericardial scaffolds by residual DNA content analysis and the effect of glutaraldehyde crosslinking on tissue holding capacity and bioresorption of decellularised bovine pericardial scaffolds using in vitro enzymatic degradation properties are undertaken in this study. Residual DNA content analysis confirmed that different decellularisation protocols effectively altered predictive immunogenicity values in pericardial scaffolds. Controlled crosslinking with 0.25% and 0.5% glutaraldehyde for 10 minutes significantly altered the biodegradation using collagenase enzyme.

Keywords : Bovine pericardium, Collagenase susceptibility, Crosslinking, Decellularisation