Article Details Date: 01-04-2023 12:00:00

Case Report (Published online: 01-04-2023)
DIAGNOSIS OF SHEEP ASSOCIATED MALIGNANT CATARRHAL FEVER IN BUFFALO BY HEMINESTED POLYMERASE CHAIN REACTION
Neeraja, D., Vimala Devi, B., Sireesha, G., Ratna Kumari, L. and Amarendra Kumar, R.
jivaonline, 86-89
Neeraja, D., Vimala Devi, B., Sireesha, G., Ratna Kumari, L. and Amarendra Kumar, R.: Veterinary Biological and Research Institute, Labbipet, Vijayawada-520010 Animal Husbandry Department, Andhra Pradesh.

Abstract

A case of sheep associated malignant catarrhal fever (MCF) in buffalo was observed in Badudandla village, Panyam mandal, Kurnool district of Andhra Pradesh. The buffalo was reported to have suffered from high temperature, severe conjunctivitis and hyper salivation before death. Nasal swabs, spleen and lung tissue were received from Animal Disease Diagnostic Lab, Kurnool district. The samples were subjected to MCF heminested PCR using 556- 755 and 556 -555 primer pairs. All the three samples were found positive for MCF with specific amplicon size of 422 bp and 238 bp in outer and inner PCR, respectively confirming the presence of sheep associated Malignant Catarrhal Fever. The samples were also tested for infectious bovine rhinotracheitis (IBR) by Real Time PCR kit and found negative.

Keywords : Heminested PCR, Malignant Catarrhal Fever, Ovine Herpes Virus -2 (OvHV-2)

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Article history: Received: 16-12-2022, Accepted : 13-03-2023, Published online: 01-04-2023

Corresponding author: Neeraja, D.