Abstract

Antimicrobial resistance (AMR) is a serious global dispute that is threatening both human and animal health in the current millennium. As an outcome, it violates the monetary stability of millions of rural farmers as well as the nation’s food security. The foremost predecessor for the formation of drug-resistant superbugs is the acquisition of newer resistant strategies by microorganisms against the conventional antibiotics. In light of the projected rise in drug resistance, essential oils seem to be a feasible candidate for existing antibiotics. Bovine mastitis milk sample was found to contain both gram-negative and gram-positive bacteria. Isolated bacteria were identified by its growth in selective and differential medium as well as with various biochemical tests specific to the pathogen. By using the Kirby-Bauer disc method, its multiple antibacterial resistance was tested. Minimum inhibitory concentration (MIC) and disc diffusion assays were done using geraniol as a test substance and results were compared with nalidixic acid. Inhibition was observed at different concentrations. This provides preliminary evidence that geraniol might be a superior alternative for treating bacteria that are resistant to medications.

Keywords : Essential oil, antimicrobial resistance, Mastitis, Geraniol