Abstract

Plants and their derivatives were used as therapeutic agents since ancient times. The magnificent uses of phytochemicals in therapy make them strong pillars in medicine. Their easy availability and less adverse effects make it as a choice for the treatment. The uses of plant compounds in combination with antibiotics to combat resistance are being explored. This study was designed to assess the resistance modulating potential of methanolic leaf extract of Saraca asoca or Indian Ashoka against tetracycline resistant E. coli isolates from clinical samples. Phytochemical analysis of the leaf extract was done qualitatively by colour reaction. The extract was also subjected to GC-MS analysis. The resistance modulating potential was assessed by Kirby-Bauer disc diffusion assay employing various concentrations of extract along with tetracycline. The phytochemical analysis revealed the presence of alkaloids, tannins, flavonoids, and saponins in the extract. There was a dose dependent increase in the zone of inhibition in the disc diffusion assay. Highest dose could significantly overcome the tetracycline resistance. Hence it can be concluded that S. asoca can be used as a promising agent to combat antibiotic resistance.

Keywords : Saraca asoca, Escherichia coli, tetracycline, antimicrobial resistance, Kirby-Bauer Disc Diffusion assay